Each department at the BPRC is involved in the development of alternatives for animal studies to fulfill the three R's in animal research, e.g. the in vitro culture of malaria liver stages. Furthermore, there is a separate Alternatives department. The unit develops in vitro technologies for specific cell types or organs as an alternative for research with live animals. A second research line is the identification of new adjuvants, e.g. an animal friendly version of the often irreplaceable complete Freund's adjuvant by separating immune stimulatory and hazardous constituents. This unit has developed various in vitro and ex vivo models. New in vitro/ex vivo assays can be developed, depending on the questions and requests.

Selected References

Statins amplify TLR-induced responses in microglia via inhibition of cholesterol biosynthesis.
Van Der Putten C, Kuipers HF, Zuiderwijk-Sick EA, Van Straalen L, Kondova I, Van Den Elsen PJ, Bajramovic JJ.
Glia. 2012 Jan;60(1):43-52.

Alternative methods for the use of non-human primates in biomedical research.
Burm SM, Prins JB, Langermans J, Bajramovic JJ.
ALTEX. 2014;31(4):520-9.

Inflammasome-induced IL-1β secretion in microglia is characterized by delayed kinetics and is only partially dependent on inflammatory caspases.
Burm SM, Zuiderwijk-Sick EA, 't Jong AE, van der Putten C, Veth J, Kondova I, Bajramovic JJ. J Neurosci. 2015 Jan 14;35(2):678-87.